delisted - One.Step RT-PCR Master Mix with UNG and dUTP instead dTTP

Features:

• The kit contains all reagents required for RT-qPCR in a set to ensure fast and easy preparation with a minimum of pipetting steps. Just add template, primers. That's all.
   
• The One.Step RT-PCR with UNG Kit is highly sensitive: less than 10 pg to 1 µg total RNA or < 1 pg to 20 ng poly(A) RNA (mRNA) can be detected when using highly expressed transcripts

Description:
The enzyme mix is based on a new formulated reverse transcriptase, a Hot-Start Polymerase (mAB), UNG and RNase Inhibitor. The set is providing increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments. The reaction buffer includes extrapure dNTPs (dUTP), and reaction enhancers.

Principal:
In the RT-step the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. The hot-start polymerase activity is blocked.
In the first cycle of the PCR step the Hot-Start DNA polymerase activity is switched on and it synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template.

Platforms: The Kit is suitable for all block-based Thermocycler. Stringent Quality Tests on ABI StepOne plus PCR Cycler

Components  of Maximo.OneStep RT-qPCR:
Enzyme-mix: HotStart Taq Polymerase with mAB, Reverse Transcriptase, RNase Inhibitor and enhancers, Reaction buffer, UNG, dNTPs incl. dUTP, and stabilizers

RNase-free water

Storage:
@ -20°C, avoid frequent thawing and freezing,

Transport:
the product will be shipped with "blue ice"

Manufactured and quality-controlled in accordance with DIN ISO 9001 and 14001

The RT-Mastermix with Uracil-N-Glycolase and dUTP eliminates the risk of contamination of DNA from former PCR reactions.

Protocols / Manuals
The datasheet includes detailed protocols for:

1.) RT-PCR assay without sample denaturation (as a standard RNA/primer combinations)

2.) RT-PCR assay with sample denaturation (RNA/primer with a high degree of secondary
structure)