RT-PCR Reverse Transcription - Kits, Enzymes and Mixes
Reverse transcriptases and cDNA synthesis products and mastermixes, including a Master Mix for one-Step RT-PCR validated for COV-19 virus detection, designed to achieve reliable and consistently high yields of full-length cDNA and excellent results.
Reverse transcriptase, also called RNA-directed DNA polymerase, is an enzyme encoded from the genetic material of retroviruses that catalyzes the transcription of retrovirus RNA (ribonucleic acid) into DNA (deoxyribonucleic acid). Reverse transcriptase is central to the infectious nature of retroviruses, several of these cause disease in humans, including human immunodeficiency virus (HIV).
RT-qPCR Mastermixes
One.Direct.Step RT-qPCR Kit for Probe or with SybrGreen (ENHANCED: 2nd Generation)
Direct RT-PCR: Amplification directly from swaps, tissue, blood or cell material - Safe time, increase your result.
The Kits are available in the combination:
Direct RT-PCR-ProbesMastermix:
Direct RT-PCR-Mastermix with SybrGreen
One.Step RT-qPCR Enzyme-Mix for TaqMan/Probes
The MaximoOne.Step RT-qPCR Kit is designed for quantitative real-time analyses of RNA templates using Dual Labelled Fluorescent Probes
One.Step RT-qPCR Enzyme-Mix for Probes with UNG (validated for COVID-19 testing)
The MaximoOne.Step RT-qPCR Kit is designed for quantitative real-time analyses of RNA templates using Dual Labelled Fluorescent Probes. The kit uses UNG and dUTP instead dTTP to stop carry-over contamination
Targets from <1 pg mRNA or 10 pg total RNA are detectable with the One.Step RT-PCR Mastermixes from GeneON
The RT-qPCR Kits are designed for performing highly sensitive and specific RT-PCR convenient in single tubes
One.Step RT-qPCR Kit (5X) "Perfect"
The One.Step RT-qPCR Kit (5X) "Perfect" with separate ROX dye contains is an advanced Kit with 5x PCR and 20x RT Mix for high specificity and low background.
The kits contain all reagents required for RT-qPCR to ensure fast and easy preparation with a minimum of pipetting steps. The premium quality enzyme mixture and the optimized reaction buffer containing extra-pure dNTPs ensure superior real time PCR results
RT-qPCR-Master Mixes and Kits - One-step RT-PCR is the most used application to process samples at a time, for one or a few targets per sample. Using Direct RT-PCR Mixes safe your time.
The Single-Step RT-qPCR Kits are manufactured for quantitative real-time analyses of RNA templates using Dual Labelled Fluorescent Probes or SybrGreen. The enzyme mixture contains a genetically engineered reverse transcriptase enzyme with enhanced thermal stability and a Polymerase blocked by monoclonal Antibodies.
It provides increased specificity and high cDNA yield, as well improved efficiency for highly structured and long cDNA fragments.
Mastermixes with SybrGreen
RT-PCR Master Mix with SybrGreen no ROX
The superior Mastermix is based on a genetically engineered reverse transcriptase; for improved efficiency; structured and long cDNA fragments; contains SybrGreen as intercalating dye
RT-PCR Master Mix with SybrGreen High ROX
Great Mastermix for RT- real time PCR based on a genetically engineered reverse transcriptase; for improved efficiency, thermostability and specificity; structured and long cDNA fragments; contains SybrGreen as intercalating dye and ROX as reference
highly structured and long cDNA fragments; includes ROX as Reference dye
RT-PCR Master Mix with SybrGreen with dUTP/UNG, no ROX
The mastermix contains dUTP and UNG to avoid cross contamination
RT-PCR Master Mix with SybrGreen with dUTP/UNG, High ROX
The mastermix contains dUTP and UNG to avoid cross contamination
"Classics" Enzymes and components for RT-PCR
First Strand cDNA PLUS Synthesis Kit
The Maximo First Strand cDNA Synthesis Set includes all components for a simple and flexible first strand cDNA synthesis
The gDNA Removal Kit is designed for removal of contaminating gDNA from RNA prior to reverse transcription
MMLV-Reverse Transcriptase: The enzyme that synthesizes a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single stranded DNA as a template
Ribonuclease (RNase) Inhibitor
RNase Inhibitor is a recombinant human placental protein which specifically inhibits ribonucleases (RNases) A, B and C
Standard primer for RT-PCR
Mixture of single-stranded random hexanucleotides
Contact
GeneOn GmbH
Paracelsusstrasse 10
67071 Ludwigshafen am Rhein
Deutschland / Germany
Phone: +49-621-5720 864
Fax: +49-621-5724 462
Shop-Terms
AGB / Businesss Condtitions / Freight Costs / Frachtkosten für Deutschland
See Categories
- DNA/RNA Extraction
- Real-time PCR
- RT-qPCR - Master Mixes
- PCR / DNA Amplification
- RT-PCR - Reverse Transcription
- Nucleotides, dNTPs, Primers
- Marker / Gel Staining.
- Enzymes and Fine chemicals
- Fluorescent Dyes / supplements
- DNA/Ladders/Standards
- PCR-96 plates and tools, Tips, Tubes, Filtration
- Restriction Endonucleases
- Devices and Tools
News & Informations
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GENEON GmbH - Germany - Paracelsusstrasse 10 - 67071 Ludwigshafen - www.geneon.net - info(at)geneon.net - Tel: 0621-5720864