RT-PCR Reverse Transcription: Datasheet collection-page
AMV reverse transcriptase
Rnase Inhibitor
random Primer
Lyophilized Mastermix for RT-PCR
Description
RT-PCR is used to amplify double-stranded DNA from single-stranded RNA templates. In the RT step the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. In the first cycle of the PCR step Taq DNA polymerase synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. During subsequent rounds of cycling the DNA polymerase exponentially amplifies this double-stranded DNA template.
LYO RT-PCR Mastermix is designed for performing maximum sensitive and specific RT-PCRs convenient in single tubes. The enzyme mix is based on a genetically engineered RT polymerase with enhanced thermal stability resulting in an increased specificity, higher cDNA yield and an improved efficiency for highly structured and long cDNA fragments.
The lyophilized Mastermix contains all reagents required for successful RT-PCR (except template and primer) in one tube to ensure fast and easy preparation with a mi
RT-PCR is used to amplify double-stranded DNA from single-stranded RNA templates. In the RT step the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. In the first cycle of the PCR step Taq DNA polymerase synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. During subsequent rounds of cycling the DNA polymerase exponentially amplifies this double-stranded DNA template.
LYO RT-PCR Mastermix is designed for performing maximum sensitive and specific RT-PCRs convenient in single tubes. The enzyme mix is based on a genetically engineered RT polymerase with enhanced thermal stability resulting in an increased specificity, higher cDNA yield and an improved efficiency for highly structured and long cDNA fragments.
The lyophilized Mastermix contains all reagents required for successful RT-PCR (except template and primer) in one tube to ensure fast and easy preparation with a mi
Informations
MMuLV Revers transcriptase
oligo dt 15
First Strand cDNA PLUS Synthesis Kit
Features:
for synthesis of cDNA from 100 bp up to 10 kb length
higher cDNA yield and greater efficiency for full length cDNA synthesis compared with standard M-MLV RT
increased thermostability
Description:
The First Strand cDNA Plus Synthesis kit contains all reagents for first strand cDNA synthesis and offers a simple handling with high flexibility. A genetically engineered version of M-MLV Reverse Transcriptase with no RNase H activity is a RNA-directed DNA enzyme that synthesizes a complementary DNA strand initiating from a primer using single-stranded RNA or DNA as template.
Applications:
- Synthesis of highly structured and long cDNAfragments
- extremely sensitive and highly specific RTPCR
- DNA labeling
for synthesis of cDNA from 100 bp up to 10 kb length
higher cDNA yield and greater efficiency for full length cDNA synthesis compared with standard M-MLV RT
increased thermostability
Description:
The First Strand cDNA Plus Synthesis kit contains all reagents for first strand cDNA synthesis and offers a simple handling with high flexibility. A genetically engineered version of M-MLV Reverse Transcriptase with no RNase H activity is a RNA-directed DNA enzyme that synthesizes a complementary DNA strand initiating from a primer using single-stranded RNA or DNA as template.
Applications:
- Synthesis of highly structured and long cDNAfragments
- extremely sensitive and highly specific RTPCR
- DNA labeling
Contact
GeneOn GmbH
Paracelsusstrasse 10
67071 Ludwigshafen am Rhein
Deutschland / Germany
Phone: +49-621-5720 864
Fax: +49-621-5724 462
Shop-Terms
AGB / Businesss Condtitions / Freight Costs / Frachtkosten für Deutschland
See Categories
- DNA/RNA Extraction
- Real-time PCR
- RT-qPCR - Master Mixes
- PCR / DNA Amplification
- RT-PCR - Reverse Transcription
- Nucleotides, dNTPs, Primers
- Marker / Gel Staining.
- Enzymes and Fine chemicals
- Fluorescent Dyes / supplements
- DNA/Ladders/Standards
- PCR-96 plates and tools, Tips, Tubes, Filtration
- Restriction Endonucleases
- Devices and Tools
News & Informations
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GENEON GmbH - Germany - Paracelsusstrasse 10 - 67071 Ludwigshafen - www.geneon.net - info(at)geneon.net - Tel: 0621-5720864