MspR9 I - CC↑NGG - GGN↓CC

Activity in SEBuffers:

SEBuffer B       50 -   50%

SEBuffer G       50 -  75%

SEBuffer O              100%

SEBuffer W       50 -  75 %

SEBuffer Y        50 -  75%

SEBuffer ROSE       100%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 37°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoetyanol; 100 μg/ml BSA; and 50% glycerol;

Store at: -20°C.

Ligation: After 2-fold MspR9 I overdigestion >5% of the DNA fragments can be ligated and recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.

Reagents supplied with enzyme: 10 X SE-buffer O

Methylation sensitivity: Blocked by overlapping Dcm methylation (CmCWGG): CCAGG and CCTGG.

Inactivation: 20 minutes under 80°C