Activity in SEBuffers:

SEBuffer B       50 - 75%

SEBuffer G       75 -100%

SEBuffer O       10 - 25%

SEBuffer W       25 - 50%

SEBuffer Y           100%

SEBuffer ROSE    100%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 37°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 200ug/ml BSA; 1mM DTT; 50% glycerol;

Store: at -20°C.

Ligation: After 5-fold overdigestion with enzyme about 5% of the DNA fragments can be ligated and recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 6 u.a. of enzyme for 16 hours at 37°C.

Reagents supplied with Enzyme: 10 X SE-buffer Y

Methylation sensitivity: not tested

Inactivation: 20 minutes under 65°C