Fal I - ↑(N)8AAGNNNNNCTT(N)13↑ - ↓(N)13TTCNNNNNGAA(N)8↓

Activity in SEBuffers:

SEBuffer B          0 - 10%

SEBuffer G        25 - 50%

SEBuffer O       75 -100%

SEBuffer W             100%

SEBuffer Y        50 - 75%

SEBuffer ROSE        70%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 37°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.

Ligation: After 3-fold overdigestion with enzyme 20% of the DNA fragments can be ligated and of these 80% can be recut. In the presence of 10%PEG ligation is better.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.

Reagents supplied with Enzyme: 10 X SE-buffer W, SAM

Methylation sensitivity: not tested

Inactivation: 20 minutes under 65°C

Notes: High enzyme concentration results in star activity

To obtain 100% activity, SAM should be added to a final concentration 0.01 mM.