Activity in SEBuffers:

SEBuffer B       75 -100%

SEBuffer G       25 - 50%

SEBuffer O             100%

SEBuffer W      50 - 75%

SEBuffer Y       50 - 75%

SEBuffer ROSE     100%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 60°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol;

Store: at -20°C.

Ligation: After 3-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 60°C.

Reagents supplied with Enzyme: 10 X SE-buffer O, BSA

Methylation sensitivity: not tested

Inactivation: 20 minutes no

Notes: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. 

Do not use BSA for long incubation.