BstPA I - GACNN↑NNGTC - CTGNN↓NNCAG

Activity in SEBuffers:

SEBuffer B       50 - 75%

SEBuffer G       25 - 50%

SEBuffer O       50 - 75%

SEBuffer W       50 - 75%

SEBuffer Y              100%

SEBuffer ROSE      100%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 65°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol.

Store: at -20°C.

Ligation: After 5-fold overdigestion with enzyme less than 5% of the DNA fragments can be ligated

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 25°C

Reagents Supplied with Enzyme: 10 X SE-buffer Y

Methylation sensitivity: not tested

Inactivation: 20 minutes no

Notes: High enzyme concentration and incubation at 65°C for 16 hours results in star activity.