Activity in SEBuffers:

SEBuffer B       50 -   75%

SEBuffer G       50 -  75%

SEBuffer O       50 - 100%

SEBuffer W              100%

SEBuffer Y        75 - 100%

SEBuffer ROSE       100%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 37°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol;

Store: at -20°C.

Ligation: After 10-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated and recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.

Reagents Supplied with Enzyme: 10 X SE-buffer W, BSA

Methylation sensitivity: not tested

Inactivation 20 minutes: No

Notes: High enzyme concentration may result in star activity.

To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. 

Do not use BSA for long incubation.