Activity in SEBuffers:

SEBuffer B            10 -  25%

SEBuffer G           25 -  50%

SEBuffer O                 100%

SEBuffer W         75 - 100%

SEBuffer Y           10 -  25%

SEBuffer ROSE          100%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 37°C

Storage conditions: 10 mM KH2PO4(pH 7.2); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.

Store: at -20°C.

Ligation: After 5-fold overdigestion with enzyme more than 95% of the Lambda DNA fragments can be ligated with T4 DNA Ligase at 16°C and 50% of these can be recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.

Reagents Supplied with Enzyme: 10 X SE-buffer O, BSA

Methylation sensitivity: Blocked by CG methylation.

Inactivation: 20 minutes under 65°C

Notes: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation. 

BspACI has a non-palindromic recognition site.