Activity in SEBuffers:

SEBuffer B             0 - 10%

SEBuffer G           10 - 25%

SEBuffer O           50 - 75%

SEBuffer W          75 -100%

SEBuffer Y           10 - 25%

SEBuffer ROSE            5%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 37°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.

Store: at -20°C.

Ligation: After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.

Reagents Supplied with Enzyme: 10 X SE-buffer 2W, BSA

Methylation sensitivity: Bsp19I cuts hemimethylated site 

5`-(5mC)CATGG-3`/3`-GGTACC-5` 

and doesn't cut methylated sites 

5`-(5mC)CATGG-3`/3`-GGTAC(5mC)-5` and

5`-(4mC)CATGG-3`/3`-GGTAC(4mC)-5`.

Inactivation: 20 minutes under 65oC

Notes: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.  Do not use BSA for long incubation.