Activity in SEBuffers:

SEBuffer B       50 -  75%

SEBuffer G             100%

SEBuffer O       50 -  75%

SEBuffer W       50 -  75%

SEBuffer Y        75 -100%

SEBuffer ROSE        75%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 37°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol.

Store: at -20°C.

Ligation: After 10-fold overdigestion with enzyme about 80% of DNA fragment can be ligated. Of these about 95% can be recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.

Reagents Supplied with Enzyme: 10 X SE-buffer G

Methylation sensitivity: not tested

Inactivation: 20 minutes under 80°C