Bpu10 I - CC↑TNAGC - GGANT↓CG

Activity in SEBuffers:

SEBuffer B       10 - 25%

SEBuffer G       25 - 50%

SEBuffer O       50 - 75%

SEBuffer W       50 - 75%

SEBuffer Y        25 - 50%

SEBuffer ROSE      100%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 37°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 50% glycerol.

Store: at -20°C

Ligation: After 5-fold overdigestion with enzyme 80% of the DNA fragments can be ligated. Of these 90% can be recut. In the presence of 10% PEG ligation is better.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 1 hours at 37°C.

Reagents Supplied with Enzyme: 10 X SE-buffer K

Methylation sensitivity: not tested

Inactivation: 20 minutes under 80°C

Notes: High enzyme concentration may result in star activity or incomplete DNA cleavage. We recommend increasing incubation time instead of using an excess of Bpu10I. The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,5.