Activity in SEBuffers:

SEBuffer B        10 - 25%

SEBuffer G        50 - 75%

SEBuffer O        50 - 75%

SEBuffer W             100%

SEBuffer Y       75 - 100%

SEBuffer ROSE      100%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 37°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol;

Store: at -20°C.

Ligation: After 20-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.

Reagents Supplied with Enzyme: 10 X SE-buffer W

Methylation sensitivity: not tested

Inactivation: 20 minutes under 65°C

Notes: The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,13. BmtI cleaves linear plasmid DNA at a rate 5 times higher than supercoiled plasmid DNA.