Activity in SEBuffers:

SEBuffer B       25 -50%

SEBuffer G      10 -25%

SEBuffer O      10 - 25%

SEBuffer W      25 - 50%

SEBuffer Y            100%

SEBuffer ROSE      50%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 55°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol;

Store at -20°C.

Ligation:  After 3-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 6 u.a. of enzyme for 16 hours at 55°C.

Reagents Supplied with Enzyme: 10 X SE-buffer Y

Methylation sensitivity: Not blocked by dcm methylation: CCWGG

Inactivation: 20 minutes under 65°C

Notes: At 37°C activity is about 10% from maximum.