Activity in SEBuffers:

SEBuffer B           25 -  50 %

SEBuffer G           50 -  75%

SEBuffer O           50 -  75%

SEBuffer W                100%

SEBuffer Y           10 -  25%

SEBuffer ROSE          100%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 50°C

Storage conditions: 20 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 50% glycerol;

Store at: -20°C.

Ligation: After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 50°C.

Reagents supplied with enzyme: 10 X SE-buffer W, BSA

Methylation sensitivity: not tested

Inactivation: 20 minutes under 80°C

Notes: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.