- The Master mix contains dTTP

- It contains EvaGreen as fluorescent dye

- The qPCR / RT-PCR Mastermix EVA 1 is ready-to-use and is              optimized for high specificity and sensitivity because of optimized        reaction buffer

- Easy to use because ready-to-use Master Mix

- The Master Mix can be used with ROX as reference dye (1x

  concentrated)

Applications:

- Detection and quantification of DNA and cDNA targets

- Profiling gene expression

- Microbial detection

- Viral load determination

- Analysis of SNPs, mutations and methylations in PCR amplicons

Concentration: The Master mix is 2x concentrated

List of components:
Hot-Start Polymerase for qPCR, dATP, dCTP, dGTP, dTTP (0,5mM each), optimized reaction buffer with  EvaGreen and 4mM MgCl2, stabilizers and enhancers, PCR-grade water

Transportation: with blue ice

Storage: at 4°C for 3 months, at -20°C for more than 12 months,

Note: protect from Light

Recommended ROX mixture:

HighROX (0.6-1 µl per 50 µl of total reaction volume): Applied Biosystems: 7000, 7300, 7700, 7900HT Fast, StepOne, StepOnePlus.

LowROX (0.6-1 µl per 50 µl of total reaction volume): Applied Biosystems: 7500, Stratagene: Mx3000P, Mx3005P, Mx4000P.

Usage:

- vortex all solutions carefully before using and before PCR
- may you add the enzyme mix after Template DNA
- an individual optimization of annealing temperature may be
  necessary for new combinations of primers and Template DNA

General Thermo-Cycler protocol for qPCR / RTD-PCR Master mix:

Note: working with EvaGreen just select the optical setting for FAM or SYBR Green at the cycler

Note: 
- an individual optimization of annealing temperature may be                 necessary for new combinations of primers and Template DNA

- up to 10x repeated freeze-thaw cycles do not influence the activity of this product