Multiplex qPCR Mastermix (No ROX, Low ROX, High ROX)

Features:
- Excellent Multiplex Master for assays of 4 and more

  targets.

- Hotstart Taq with mAB and all components for succesfull

  multitarget PCR

- Setting up at roomtemperature; optimized for block based
  instruments

- 2-times concentrated; 2x1,25 ml Mastermix

Available mixtures:

- NO-ROX (product code: M100-200; M100-1000)

- LOW-ROX (product Code: M110-200, M110-1000)

- HIGH-ROX: (product code: M120-200, M120-1000)

Applications:
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination

Compatibility:
Block based PCR-Cycler

Description:
The PCR-Master Mix contains all reagents required for qPCR (except template and primer) in a premixed 2x concentrated ready-to-use solution.

The high specificity and sensitivity of the mix is achieved by an optimized hot-start polymerase.

Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formations at low temperatures during PCR setup.
The mix offer dUTP instead of dTTP.

Concentration: The Mastermix is 2x concentrated

List of components qPCR / MultiplexMaster mix:
Hot-Start Polymerase for qPCR, dATP, dCTP, dGTP, dUTP, ROX (depending on the product-code), optimized reaction buffer with KCl, Ammoniumsulfate and MgCl₂, stabilizers and enhancers,

separate Tube: PCR-grade water

Transportation: with blue ice

Storage: @ 4°C up to 3 months; @ -20°C for more than 12 months,

Note: protect from Light when ROX is used