Discontinued: Replaced by Y220

Features:

 - Enzyme with hot start capability increases reaction specificity and
   sensitivity, optimized

- DFS-Taq PLUS DNA polymerase activation requires not more than 5
  min heating

- High selectivity and reaction yield

- The mix is colored for easy pipetting

- Reduced preparation time

Applications:

- Real-time PCR with intercalating dye SYBR Green I

- Conventional PCR

- High-throughput PCR

- Genotyping

- For detection of bacterial DNA

Description

Bio-Star qPCR-Mastermix SYBR Blue (2×) is developed for quantitative real-time PCR with fluorescent dye SYBR Green I. The Mastermix contains all components, except template and primers, for successful PCR.

- The PCR-mix is optimized for efficient and reproducible hot-start real-time PCR of genomic, plasmid and viral DNA samples.

- The solution contains substances that increase half-life and
  processivity of DFS-Taq PLUS DNA polymerase by enhancing its
  stability during PCR.

- It includes components that influence primer annealing temperature
  and characteristics of template melting thus enabling to increase the
  specificity of PCR and use templates with complicated structure.

- DFS-Taq Plus DNA polymerase is inactive at room temperature
  because of monoclonal antibodies.

- The inert dye allows control when using multi-well plates. Use of the kit
  saves time and minimizes contamination risk due to reduced number of
  pipetting steps.

Components and Mixture

2X Bio-Star qPCR-Mastermix SYBR Blue contains:

- 100 mM Tris-НCl (рН 8.5 at 25 °С), 100 mM KCl, 0.4 mM each of dNTP,
   3 mM MgCl2, 0.06 U/µl DFS-Taq DNA Polymerase, 0.025% Tween 20,
   stabilizers and enhancers, SYBR Green I, Blue inert-dye, which does
   not interfere the PCR in a real-time Cycler

- Tube of MgCl2 100 mM

- Water Mol.Bio Grade 2 x 1,25 ml

Storage and transportation: at -20 °С; not more than 50 thawing-freezing cycles. shipping with blue ice or at room temperature

Storage terms: up to 24 mounts

Exodeoxyribonuclease activity: DNA was stable after incubation of 1 µg fragment of phage lambda DNA in the presence of 25 µl of Bio-Star qPCR Mastermix (2×) in 50 µl reaction solution at 37 °C and 70 °C for 4 h.

Test for bacterial DNA contamination

Test for Hot -Start ( we test activity with or without MAB at various temperatures

Exodeoxyribonuclease activity

DNA was stable after incubation of 1 μg fragment of phage lambda DNA in the presence of 25 μl of BioMaster HS-qPCR (2×) in 50 μl reaction solution at 37 °C and 70 °C for 4 h.

Ribonuclease activity

Absence of ribonuclease activity was confirmed after incubation of 1 μg of 5’-[P32]-labeled RNA fragment in the presence of 25 μl of BioMaster HS-qPCR (2×) in 50 μl reaction solution at 37 °C for 4 h.

Stability:

One month at room temperature does not reduce PCR efficiency

Amplification protocol

1. Defrost the reaction mixture and stir thoroughly.

2. Add the following components into the thin-wall PCR tubes considering the final volume of a reaction mixture equal to 50 μl:

Dear customer, GeneON likes to send free samples to convince the valued customer about the quality. Please understand that the shipping costs may be very high to some destinations. That is the reason why we cannot assure to fulfil all sample requests. Sorry for that, please understand. You may also set an inquiry/order directly by e-mail .