Notes: Protein Marker / Protein Standard / SDS-PAGE / Western Blot / Protein Electrophoresis
Description/Preparation:
The Protein ladder EXtended contains 13 pre-stained proteins that resolve into sharp, tight bands in the range of 3,5-245 kDa. The Protein Ladder allows to monitor molecular weight separation
during electrophoresis, estimate molecular weights. The marker is ready-to-use. There is no need to boil. Easy to identify: Proteins are
covalently coupled with a blue chromophore except for two reference bands; green band at 25 kDa and red band at 75 kDa.
Applications
• Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis.
• Monitoring of protein transfer onto membranes during Western blotting.
• Sizing of proteins on SDS-polyacrylamide gels and Western blots.
Usage of Protein ladder:
Mini gel application: 5 μl/well; 2.5 µl per well blots
Standard gel application: 10 μl/well; 5 µl per well for blots
Number of bands: 13 5, 11, 27, 20, 25, 35, 48, 63, 75, 100, 135, 180, 245 kDa
Loading:
Loading Denaturing Polyacrylamide gels
(SDS-PAGE):
- Thaw marker at room temperature or heat at 37 – 40 °C for a few minutes. Do not boil!
- Vortex gently to ensure the solution is homogeneous and load the ladder on SDS-
polyacrylamide gel
- 5 μl per well for mini-gels, 2.5 μl per well for blots
- 10 μl per well for large gels, 5 μl per well for blots
1 ml marker is sufficient for 200 mini gels or 100 standard gels.
It is recommended to divide the marker into aliquots to avoid contamination of the stock solution.
It is recommended to divide the marker into aliquots to avoid contamination of the stock solution.
Note:
Protein ladder PS11 is optimized for runs on 15 % SDS
polyacrylamide gels. 4 to 12 % gels may cause
proteins with low molecular weights to migrate with the dye front. On 12 to 15 % and gradient gels all bands are visible.
Quality Control
Tested in SDS-polyacrylamide gel electrophoresis and Western blotting.
Storage: at -20°C or at room
temperature
Shipment: on blue ice
Protein Molecular Weight Marker "EXtended" (prestained) is ideal for precise sizing of proteins by SDS-PAGE and for Western blotting.
* availibility of sample size may be limited
Final price excl. shipping costs3
Dear customer, GeneON likes to send free samples to convince the valued customer about the quality. Please understand that the shipping costs may be very high to some destinations. That is the reason why we cannot assure to fulfil all sample requests. Sorry for that, please understand. You may also set an inquiry/order directly by e-mail .