Description/Preparation:
Protein ladder PS11 contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kDa. The Protein Ladder allows to monitor molecular weight separation during electrophoresis,
estimate molecular weights The marker is ready-to-use. There is no need to boil. Easy to identify: includes the ~10, ~40 and ~90 kDa reference bands coupled with an blue dye
Applications
• Monitoring of protein migration during SDS-
polyacrylamide gel electrophoresis.
• Monitoring of protein transfer onto membranes
during Western blotting.
• Sizing of proteins on SDS-polyacrylamide gels
and Western blots.
Usage:
Mini gel application: 5 μl/well; 2.5 µl per well blots
Standard gel application: 10 μl/well; 5 µl per well for blots
Number of bands: 11 10.5, 14, 22, 29, 42, 51, 62, 70, 95, 130, 175 kDa
Loading:
Loading Denaturing Polyacrylamide gels (SDS-PAGE):
- Thaw marker at room temperature or heat at 37 – 40 °C for a few minutes. Do not boil!
- Vortex gently to ensure the solution is
homogeneous and load the ladder on SDS-
polyacrylamide gel
- 5 μl per well for mini-gels, 2.5 μl per well for blots
- 10 μl per well for large gels, 5 μl per well for blots
1 ml marker is sufficient for 200 mini gels or 100 standard gels.
It is recommended to divide the marker into aliquots to avoid contamination of the stock solution.
Note:
Protein Marker PS11 is optimized for runs on 15 % SDS polyacrylamide gels. 4 to 12 % gels may cause
proteins with low molecular weights to migrate with the dye front. On 12 to 15 % and gradient gels all bands are visible.
Quality Control
Tested in SDS-polyacrylamide gel electrophoresis and Western blotting.
Storage: at -20°C
Shipment: on blue ice
Protein Molecular Weight Marker PS11 (prestained) is ideal for precise sizing of proteins by SDS-PAGE and for Western blotting.
* availibility of sample size may be limited
Final price excl. shipping costs3
Dear customer, GeneON likes to send free samples to convince the valued customer about the quality. Please understand that the shipping costs may be very high to some destinations. That is the reason why we cannot assure to fulfil all sample requests. Sorry for that, please understand. You may also set an inquiry/order directly by e-mail .