Taq DNA Polymerase blue ready-to-load

Features:
Maximo Taq-Blue DNA Polymerase provides robust PCR performance in a wide range of PCR applications and different templates.  After PCR reaction the enzyme can be loaded to agarose gel, no dye and DNA loading buffer is needed. The enzyme is time- and cost saving, because it includes dye and loading buffer, already.

Applications:
- Standard / General PCR with visible control
- High-throughput PCR
- Primer extension
- Gene mutation
- T/A cloning

Description: 
Maximo Tag-Blue DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-stranded specific 5´→3´ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94kDa.

Concentration:  1 u/µl

Unit definition:
One unit incorporates 10 nmol of deoxyribonucleotide into acid-precipitation material in 30min at 74 degree

Storage Buffer:
25mM Tris-HCl (pH8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% NP 40, 0.5% Tween 20, in blue loading buffer

Reaction Buffers supplied with the enzyme:

10X Buffer I:  500mM KCl, 100mM Tris-HCl, pH 9.0, 1% Triton X-100, 15mM MgCl₂
MgCl₂: 100 mM
 
Transportation: on blue ice

Storage: at -20°C for 12 months

Quality control: 
- PCR with various templates – genomic DNA, Phage Lambda DNA
- 2 kb DNA amplification from 50 ng DNA
- batch variation and level of bacterial DNA contamination

Note: 
- vortex all solutions carefully before using
- dispense all reagents on ice
- add the enzyme after Template DNA
- may you have to optimize the MgCl₂ concentration for best result

Taq DNA Polymerase General Thermo-Cycler protocol:

Dear customer, GeneON likes to send free samples to convince the valued customer about the quality. Please understand that the shipping costs may be very high to some destinations. That is the reason why we cannot assure to fulfil all sample requests. Sorry for that, please understand. You may also set an inquiry/order directly by e-mail .