Features:
- 10-15 fold lower mutation rate than Taq DNA Polymerase 
- high fidelity allele-specific amplification of DNA fragments
- high specificity with lowest background AS-PEX and AS-PCR
- Hot-Start activity for less primer dimers
- only 5'-3' polymerase activity, lack of 5’-exonuclease activity

Applications:
- High specific PCR
- Single Nucleotide Polymorphism (SNP)
- Multiplex PCR
- Real-Time PCR with intercalation dyes
- high fidelity dNTPs and ddNTPs
- Mini-Sequencing, SNP-genotyping

Description:
SNPase is Taq DNA Polymerase  with unique N-terminal deletion and proprietary amino acids substitutions introduced into the active center of the enzyme. This modification causes dramatic increase of sensitivity of the enzyme to mismatches at 3’-end of the primer. Consequently , non-perfect annealing of the primers does not result in unspecific amplicons formation. This enzyme has only 5'-3' polymerase activity and is recommended for SNP genotyping by allele-specific PCR (AS-PCR), allele-specific primer extension (AS-PEX) and minisequencing

procedures.

Unit definition:
One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid-insoluble
form in 30 minutes at 72°C.

Concentration:
20-25 u/µl

Reaction Buffer supplied:
5X Reaction buffer without MgCl₂
MgCl₂ 100 mM

Note:
- optimal MgCl₂ concentration: 3.0 -3.5 mM in the 1X reaction mixture
- higher MgCl₂ concentrations results in higher yield (up to 4.5 mM)
- lower MgCl₂ (2.5 mM) results in higher specificity
- DNA fragments up to 400 bp from Human genomic DNA and 500 bp
  from Phage-DNA

Minisequencing SNP genotyping with SNPase DNA Polymerase can be carried out by the procedure described in *:

* Reference for minisequencing protocol: Lovmar L, Fredriksson M, Liljedahl U, Sigurdsson S, Syvänen AC.
Quantitative evaluation by minisequencing and microarrays reveals accurate multiplexed SNP genotyping of whole genome amplified DNA. Nucleic Acids Res. 2003;31:e129.