Monoclonal Antibody / Mixture of Antibodies to Taq DNA Polymerase / Monoclonal Antibody to Pfu DNA Polymerase: m-Anti-Taq

Features:
- Anti-Taq (monoclonal antibody to Taq DNA polymerase) inhibits the
  reaction of Taq DNA Polymerases at room temperature
- Ideal as enhancer for PCR reactions or for "homemade" Hot-Start
  Polymerase

By preventing Taq polymerase activity until the denaturation step of PCR is underway, nonspecific amplification due to mispriming and/or formation of primer dimers during PCR assembly is prevented. As a result, experimental background is significantly decreased, and reaction specificity is increased.

Application:
Polymerase detergent/enhancer for:
- High specific amplification
- Multiplex amplification
- High sensitivity applications
- Low-copy number PCR

Unit definition:
One unit is defined as the amount required to blocks 50% activity of 1 µg of  Polymerase

By preventing Taq polymerase activity until the denaturation step of PCR is underway, nonspecific amplification due to mispriming and/or formation of primer dimers during PCR assembly is prevented. As a result, experimental background is significantly decreased, and reaction specificity is increased.

Note: The ratio units/mg of polymerase varies (up  to factor of 10). The binding rate Anti-Taq depends strongly of the polymerization region (active epitopes) of the polymerase.  The optimized mixture has to be found in empiric test and for every new lot of polymerase, again.

Storage Buffer for S131/S132
20mM Tris-HCl (pH 7.0, at 22^oC);50 mM KCl;0.1mM EDTA; 50% glycerol

Storage Buffer for S131/S132-8C1
3mM Tris-HCl (pH 8,0, @ 22^oC); 15 mM KCl; 0.1mM EDTA; 20 % Trehalose (pH 8,0, @ 22^oC);

Concentration: 2-10 mg/ml

Units/mg-ratio:
2300 units of the specific polymerase activity are equal to 1 mg of antibodies

Storage: at – 20°C

Transportation: at room temperature or with blue ice

 QC-Test: > 95% of protein in SDS electrophoresis in 15% PAAG

GeneON offers two types of Antibodies to Taq:

- Glycerol buffered

- Glycerol free buffered in Trehalose

NEW: Mixture of monoclonal Antibodies against Taq DNA Polymerase: ! CLEVER PRICE !

 !  DELISTED  ! Monoclonal Anti-Pfu DNA Polymerase (Antibodies to Pfu-DNA Polymerase)