Features:
Maximo Taq DNA Polymerase 2X-preMix provides robust PCR performance in a wide range of PCR applications and different templates. Best value in terms of cost per unit. The optimized mixture of all components reduces pipetting mistakes and ensures repeatable results - every day.

Applications for PCR Mastermix:
- Standard / General PCR
- optimized for high specifity
- High-throughput PCR, automated pipetting, or plate based PCR
- Gene mutation
- T/A cloning

Description: 
Maximo Taq DNA Polymerase 2X-Mastermix is optimized and ready-to-use mixture of all components for a successful PCR. Only your primers and your DNA Template has to be added. 
Maximo Taq DNA Polymerase 2X-preMix contains a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-stranded specific 5´→3´ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94kDa.

Concentration:  the mixture is 2X concentrated (Mg2+: 3,6 mM, 1,8 mM final)

Unit definition:
One unit incorporates 10 nmol of deoxyribonucleotide into acid-precipitation material in 30min at 74 degree

List of components:
0.1U/ul Taq DNA Polymerase, 0.4 mM dATP, 0.4 mM dGTP, 0.4 mM dCTP, 0.4 mM dTTP, 4 mM MgSO₄, 20 mM KCl, 16 mM (NH₄)₂SO₄, 20 mM Tris-HCl, pH8.8

Quality control: 
- PCR with various templates – genomic DNA, Phage Lambda DNA
- 2 kb DNA amplification from 50 ng DNA
- batch variation and level of bacterial DNA contamination
 
Transportation: on blue ice

Storage: at -20°C for 24 months

Note: 
- vortex all solutions and spin down carefully before using
- dispense on ice and spin down again
- may you have to optimize the MgCl₂ concentration for best result

Taq Premix General Thermo-Cycler protocol:

Dear customer, GeneON likes to send free samples to convince the valued customer about the quality. Please understand that the shipping costs may be very high to some destinations. That is the reason why we cannot assure to fulfil all sample requests. Sorry for that, please understand. You may also set an inquiry/order directly by e-mail .