Taq DNA Polymerase for daily PCR runs

Features:
Maximo Taq DNA Polymerase provides robust PCR performance in a wide range of PCR applications and different templates. Best value in terms of cost per unit.

Applications for Taq DNA Polymerase:
- Standard / General PCR
- Multiplex PCR
- High-throughput PCR
- Primer extension
- Gene mutation
- T/A cloning

- works in some application with hot start feature

Description Taq DNA: 
Maximo Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-strand specific 5´→3´ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94kDa.

Concentration:  5 u/µl

Unit definition:
One unit Taq polymerase from GeneON incorporates 10 nmol of deoxyribonucleotide into acid-precipitation material in 30min at 74 degree

Storage Buffer of DNA Poylmerase:
25mM Tris-HCl (pH8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% Nonident P40, 0.5% Tween 20

Reaction Buffers supplied with the enzyme:

10X Buffer I:  500mM KCl, 100mM Tris-HCl, pH 9.0, 1% Triton X-100, 15mM MgCl₂

Quality control: 
- PCR with various templates – genomic DNA, Phage Lambda DNA
- 3 kb DNA amplification from 50 ng DNA
- batch variation and level of bacterial DNA contamination
 
Transportation: on blue ice

Storage: at -20°C for 24 months

Note: 
- vortex all solutions carefully before using
- dispense all reagents on ice
- add the enzyme after Template DNA
- may you have to optimize the MgCl₂ concentration for best result

General Thermo-Cycler protocol:

Dear customer, GeneON likes to send free samples of DNA Polymerase to convince the valued customer about the quality. Please understand that the shipping costs may be very high to some destinations. That is the reason why we cannot assure to fulfil all sample requests for the polymerase. Sorry for that, please understand. You may also set an inquiry/order directly by e-mail .