Features: Superhot Taq DNA polymerase for qPCR is designed for Real-Time PCR and Hot-start PCR. A special inhibitor suppresses the reaction at room temperature until after the first denaturation step. This prevents primer-dimers and other artefacts. Using the enzyme there is no need to adjust the existing standard PCR protocol.

Applications: 
- Hot Start and real time PCR research
- Multiplex PCR
- Amplification of complex genomic and cDNA templates
- no primer - dimer and other artefacts; inactive at room temperature
- short activation time for real time PCR
- enhanced PCR sensitivity

Description:
Maximo M-Superhot Taq DNA polymerase for qPCR and Hot-Start-PCR is an optimized mixture of a high processive Taq DNA Polymerase and special inhibitors to Taq DNA for real time PCR. The enzyme is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-stranded specific 5´→3´ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94kDa. It is developed for real time PCR or as basis enzyme for  real time PCR diagnostics systems.
 
Concentration: 5 u/µl

Unit definition:
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP's into acid-insoluble form in 30 minutes at 74^oC under assay conditions:
25mM TAPS pH 9.3 at 25^oC, 50mM KCl, 2mM MgCl₂; 1mM beta-mercaptoethanol; 200µM each dATP, dGTP, dTTP and 100 µM dCTP (a mix of unlabeled and µ-[³²P]-labeled); 12.5 µg activated salmon sperm DNA in the final volume of 50 µl

Storage Buffer:
20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA; 1 mM DTT, 50 % Glycerol, 0.5 % Nonident P-40, 0.5 % Tween-20

Reaction Buffer:
Reaction buffer (10X)” incomplete” (red cap):160 mM (NH₄)₂SO₄, 670mM TrisHCl pH8,8, 0,1% Tween-20
Reaction buffer (10X) “complete” (yellow cap):160 mM (NH₄)₂SO₄, 670mM TrisHCl pH8,8, 0,1% Tween-20, 25mM MgCl₂
separate Tube: MgCl₂ (100 mM, green cap)

Transportation: Taq DNA polymerase is send on blue ice

Storage: at -20°C for 24 months or  for more than 3 months at +4°C

Activity and performance test in real time PCR, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases test

NEW Product: SuperHotTAQ DNA Polymerase Glycerol free for Lyophilization

Use your existing and optimized protocol for standard DNA polymerase. In contrast to chemically modified Taq DNA pol. where the first denaturation step needs up to 15 min, Maximo M-Superhot Taq for Real Time PCR does not need a prolonged heating or denaturation time and works excellent basis enzyme for real time PCR.

Note: 
- vortex all solutions carefully before using
- add the enzyme after Template DNA
- may you have to optimize the MgCl₂ concentration for best result

General Thermo-Cycler protocol:

Note: 
- In case of low amount of DNA template, additionally cycles may be used

Dear customer, GeneON likes to send free samples to convince the valued customer about the quality. Please understand that the shipping costs may be very high to some destinations. That is the reason why we cannot assure to fulfil all sample requests. Sorry for that, please understand. You may also set an inquiry/order directly by e-mail .