- Maximo H-SPlus Taq DNA Polymerase is “inactive” at room-temperature

- When the reaction temperature is greater than 70 °C the Polymerase
 “jumps” into action

- It is neither blocked by antibodies nor by chemically modification -> ultra-
  short activation time

- For “high-yield” Hot-Start PCR results

- Multiplex PCR

- PCR setting up at room-temperature

- As sensitive Polymerase for PCR Diagnostics research

Description:

Maximo H-SPlus Taq DNA Polymerase (recombinant from Thermus aquaticus) catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction in the presence of magnesium. It also possesses a 5'→3' polymerization-dependent exonuclease replacement activity but lacks a 3'→5' exonuclease activity. The activation of the enzyme starts immediately at 70°C and requires no increased time in heating or denaturation step. Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents theextension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.

Unit definition:

One unit is defined as the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble material in 30 min at 72°C.

Concentration: 5 u/µl

Storage buffer: 20 mM Tris-HCl, 100 mM KCl, 0.15 mM EDTA, 1 mM DTT, 0.5% Tween-20, 0.5% Nonidet P40, 50% (v/v) Glycerol, pH 8.1

GENEON OFFERS A GLYCEROL-free HS-Taq Polymerase. Please ask for product Code S410-GF.

Reaction buffers :

1.)

Reaction buffer (10X)” incomplete” (red cap):160 mM (NH4)2SO4, 670mM TrisHCl pH 8,8, 0,1% Tween-20, enhancers

2.)

Reaction buffer (10X) “complete” (yellow cap):160 mM (NH4)2SO4, 670mM TrisHCl pH 8,8, 0,1% Tween-20, enhancers, 25mM MgCl2

3.)

separate Tube: 100 mM MgCl2

Quality control

- H-SPlus Taq DNA Polymerase is free from endonucleases and primer contamination, positive PCR performance with several templates of Lambda DNA (<= 12 kb) and human placenta DNA (3kb).

Note: 
- vortex all solutions carefully before using
- add the enzyme after Template DNA
- may you have to optimize the MgCl₂ concentration for best result

General Thermo-Cycler protocol:

 Step                            Time              Temperature

 Initial denaturation        1-2                   94-95°C

 
25-30 Cycles:
 Denaturation                30 sec               94-95°C

 Annealing                     30 sec               48-68°C

 Extension                     0,5-3 min               72°C per 1kb

 Final extension               2-3 min                72°C

Note: - In case of low amount of DNA template, additionally cycles may be used 

Storage: at -20°C for 24 months, avoid frequent thawing and freezing

Transport: with blue ice or at ambient temperature depending on the destination