Light Cycler Capillaries - this product is discountinued !

Your advantages:

- no risk of breaking

- no risk of contamination

- same sensitivity

- no or less change in protocol

- about 40 to 50 % better price

- easy post sample recovery

Short Protocol: (see dataseet, too)

- Take your existing protocol for glass-capillaries from LightCycler

- Prepare your existing PCR-Mastermix. The reaction volume is from 15 –

   50 µl

- Wear hand cloves and use the transfer pin for easier handling

- Take the capillaries out the rack and pipette the PCR-Master mix and
   the otheR components into the upper part of the PC-Capillary

- For an easier handling may you put the PC-Capillary in a 1,5 ml
  reaction tube using a standard PCR rack

- Either you transfer the capillary with the 1,5 ml tube to a table top
  centrifuge (2-3 seconds) OR with a short jerk you bring the Mix down to
  the bottom.

- Note: Do not use the carousel-adapter from GeneON for any centrifuge
  system. It is not constructed for high speed centrifugation. GeneOn is
  not liable for any damage that occurs for the wrong usage of the 
  carousel in a centrifuge

- Close the capillary with the cap using the transfer pin

- Ensure that the cap close tightly and transfer the capillary the carousel
 (GeneOn Rotoradapter for Lightcycler)

Because of the larger diameter and the more thick walls of the PC-capillary it might be necessary to increase the “hold-times” for denaturation and annealing. We recommend increasing the times with about 10 seconds. If you already use longer times the maximum prolongation is 5 seconds. Depending on you GC-content it might be better be increase the denaturing temperature from 95°C to 97°C for higher efficiency.

Note: Longer denaturing times than 10-11 seconds brings not a better result

pc-Kapillare für Lightcycler