EvaGreen Fluorescent DNA Stain (100µM)

Stain for intercalation DNA, Evagreen is: Non-mutagenic, non-cytotoxic and safe to aquatic life for safe handling and easy disposal down the drain

Description:
EvaGreen Fluorescent DNA Stain is well known DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR) and HRM-analysis. Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process.
The high quantum yield, excellent stability and lowest inhibition toward PCR makes it the ideal fluorophore in real-time PCR applications and a superior replacement for the widely used SYBR-Green I dye.

The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling.

Shipping: blue ice or room-temperature

Storage Conditions: store at -20 °C and dark

Shelf Life: at least for 18 months

Supplied in 20 mM Tris-HCl pH 8.4, 0.1 mM EDTA and 0.01 % Tween-20

Concentration: 100 µM

Spectroscopic Properties: _λexc 500 nm (bound to DNA); _λem 530 nm (bound to DNA)

Stability tests / Quality Tests

EvaGreen is extremely stable in both thermally and hydrolytically, providing convenience during routine handling.

Applications:

Vortex EvaGreen® Fluorescent DNA Stain thoroughly prior to its use. Add 1 µl EvaGreen® Fluorescent DNA Stain for each 50 µl of the assay. Please note that the preparation of a master mix is crucial in quantitative PCR reactions to reduce pipetting errors. To perform the EvaGreen-based assay simply select the optical setting for SYBR® Green on the detection instrument.

EvaGreen Fluorescent DNA Stain (100µM)

Mechanism of DNA Binding

EvaGreen® DNA-binding dye is designed using a novel concept of DNA binding via “release-on-demand” mechanism (Fig. 1). The dye is constructed of two monomeric DNA-binding dyes linked by a flexible spacer. In the absence of DNA, the dimeric dye assumes a looped conformation that is inactive in DNA binding. When DNA is available, the looped conformation shifts via an equilibrium to a random conformation that is capable of binding to DNA to emit fluorescence. This chemical equilibrium provides a

unique mechanism to continuously supply the active form of the dye from the “reserve”(i.e., the dye in looped conformation), as more DNA is formed during a PCR process. Consequently, an EvaGreen® master mix can be formulated with relative high dye concentration to maximize fluorescence signal without PCR inhibition, making the mix suitable for both qPCR and HRM applications.*

* availibility of sample size may be limited

116,00 €

order / kaufen

Final price excl. shipping costs³

verfügbar / avaílable
1 - 3 days for delivery / 1 - 3 Tage Lieferzeit¹

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Datasheet Eva Green fluorescent DNA stain

Evagreen fluorescent dye

Evagreen is Environmentally safe
Non-mutagenic, non-cytotoxic and safe to aquatic life for safe handling and easy disposal down the drain.
Superior for qPCR and isothermal amplification
Far brighter than SYBR® Green I for detecting amplification due to its novel “release-on-demand” DNA-binding mechanism.
Unrivaled DNA melt curve performance
Low PCR inhibition permits the use of saturating dye concentration for maximal signal and high-resolution DNA melt analysis.
Evagreen is: Extremely stable
Stable during storage or under PCR conditions.

Serves both as a qPCR dye and a DNA gel stain
After the PCR reaction, directly visualize the PCR product on a gel using a UV transilluminator or blue light box without the need for another gel stain.
Compatible with multiplex PCR
Lack of dye migration from amplicon to amplicon enables detection of multiple PCR products by melt curves.
Useful for other applications
Currently the only qPCR dye to

Evagreen 100µM.pdf

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Material Safety Datasheet

MSDS Evagreen

MSDS F253 EvaGreen.pdf

Adobe Acrobat Document 97.6 KB

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References / Protocols / Notes / Recomendations / Tests

¹ Applies to shipping within Germany. Information about shipping policies for other countries can be found here: Payment and Delivery Information
² limitierter Sonderpreis / Special Price limited
³ Nettopreise ohne Umsatzsteuer zzgl. Frachtkosten / All prices without VAT excl. freight costs. :: MUSTER FÜR DEUTSCHLAND WERDEN KOSTENFREI GELIEFERT - AUCH WENN IM SHOP EIN PREIS AUSGEWIESEN WIRD :::

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