Proteinase K - Lyophilizate and customized solutions

Description: Proteinase K is a serine protease that exhibits a very broad cleavage specificity. The Protein with a molecular weight 28.900 kD cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. Proteinase K is not inactivated by chelating reagents such as EDTA or detergents such as SDS and is active over a wide range of pH (4-12.5).

Activity:  > 30 units/mg Lyophilizate and > 40 units/mg protein

Unit definition: Unit definition One unit is the amount of enzyme which releases at 37°C in 1 min as many folin-positive amino acids and peptides from haemoglobin as 1 μmol of tyrosine.

Features:

The protease or - its solution is a highly active and stable protease with low cutting specificity. The enzyme belongs to the group of subtilisine-related serine proteases and is strongly inhibited by PMSF.

Usage: In presence of 0.5 – 1 % SDS Proteinase inactivates DNases and RNases in eukaryotic and microbiological cell cultures. The use of Proteinase K during lysis of the cells allows the isolation of intact highly-molecular nucleic acids.

Quality:

- purified by chromatography and lyophilised

- RNases: not detectable

- DNases: not detectable

- Exonucleases: not detectable

Each batch of Proteinase K is tested for the presence of fungal and bacterial DNA. Moreover, the specific activity assay of each batch of the enzyme is performed. 

Storage:

4 °C or -20 °C for at least 24 months, shipment at ambient temperature or blue ice

Determination of the the stability of Proteinase K during long-term storage at room temperature. The tested Lot was compared to the fresh production batch in 6 month time intervals. Assay conditions described below.

Lot: tested: P040917 control LOT: current production batch

Storage buffer for Proteinase K in solution:

20 mM Tris pH 7,5, 1 mM CaCl2, 0,02% sodium acid, 50% glycerol (v/v).

Test results

Assay conditions

●      Temperature: 37 °C, A750

●      Reaction volume: 900 μl

●      Substrate Solution: 2% Haemoglobin with 6M Urea pH 7.5

●      Spectrophotometer: Eppendorf Biospectrometer

Dear customer, GeneON likes to send free samples of to convince the valued customer about the quality. Please understand that the shipping costs may be very high to some destinations. That is the reason why we cannot assure to fulfil all sample requests. Sorry for that, please understand. You may also set an inquiry/order directly by e-mail .