Description:
The Tissue viral RNA/DNA Extraction Kit is designed for rapid and efficient purification of genomic DNA from various tissue samples such as lungs etc. or swabs and nasopharyngeal swab salivia, sputum. The purification is based on the usage of denaturing agents to provide lysis of tissue cells, denaturation of proteins and subsequently release of  viral RNA/DNA. Special buffers provided in the kit are optimized to enhance the binding of DNA onto a specially-treated glass filter membrane for efficient recovery of highly pure genomic RNA/DNA.

Features Tissue Viral Nucleic Acid Extraction Kit:
- Mini-column spin technology
- No organic-based extraction required
- Highly pure genomic DNA ready to use for routine molecular biology applications such as  restriction enzyme digestion, PCR, - Southern blotting, DNA fingerprinting, etc.

The expected yield is up to 3ug, depends on elution volume and sample types (raw tissue or cooked tissue or tissue organ).

Application:
For high quality tissue RNA/DNA isolation.

As for: swabs and nasopharyngeal swab samples

1. Scrape the swabs firmly against the samples 6-7 times.

2. Cut the end of the swab stick containing the sample and place it into a 2ml microcentrifuge tube.

3. Add 550ul PBS, 50ul Proteinase K and 200ul Buffer VL1 to the sample. Mix thoroughly by vortexing.

4. For extraction of viral RNA, add 215ul of Buffer VL2 containing Carrier RNA. Mix homogeneously by pulsed-vortexing. Incubate at 65C for 30 min. Centrifuge at maximum speed for 1 min. Transfer supernatant into a new microcentrifuge tube. Continue step 2 (Addition of ethanol) stated at GF-TRD manual.

As for: salivia, sputum samples

1. Collect 1.5ml saliva and sputum into a clean 15ml centrifuge tube containing 6ml PBS. Mix thoroughly by vortexing.

2. Centrifuge at 2000 xg for 5 min. Discard the supernatant and resuspend the pellet in 180ul PBS.

3. Transfer the samples into a clean 1.5ml microcentrifuge tube.

4. Add 50ul Proteinase K and 200ul Buffer VL1 to the sample. Mix thoroughly by vortexing.

5. For extraction of viral RNA, add 215ul of Buffer VL2 containing Carrier RNA. Mix homogeneously by pulsed-vortexing. Incubate at 65C for 30 min. Centrifuge at maximum speed for 1 min. Transfer supernatant into a new microcentrifuge tube. Continue step 2 (Addition of ethanol) stated at GF-TRD manual.

Note:
Tissue Viral Nucleic Acid samples vary in the number of cells depending on age, type of tissue and origin. When processing samples, do not use more than the recommended starting material as excessive number of cells will overload the column. This would result in reduced yield and purity. We recommend weighing the tissue samples before starting to ensure optimum yield and purity is obtained. Liver and spleen are very high in protein and RNA content. Thus, when isolating genomic DNA from these sources, use only up to 15mg of the sample.

Dear customer, GeneON likes to send samples to convince the valued customer about the quality. Please understand that the shipping costs may be very high to some destinations. That is the reason why we cannot assure to fulfil all sample requests. Sorry for that, please understand. You may also set an inquiry/order directly by e-mail .