Features:
λ DNA is used as one of the substrates in restriction enzymes research and for testing of restriction endonucleases activity.

Description/Preparation: 
The double stranded DNA is isolated from bacteriophage lambda (cl857 ind1 Sam7). The molecular weight is 31.5 x 10⁶ daltons and it is 48,502 base pairs in length.
The phage is isolated from the heat inducible lysogen E. coli λ cl857 S7 by gel filtration. The DNA is isolated from the purified phage by phenol/chloroform extraction and dialyzed against 10 mM Tris-HCl (pH 7.4) and 1 mM EDTA.

The product is methylated.

Concentration:    0.2-0.5 µg/µl

Storage Buffer:  10 mM Tris-HCl (pH 7.4), 1 mM EDTA.

Quality control: Gel analysis for purity. EcoRI and HindIII fragmentation patterns.
 
Transportation: Shipped on blue ice or room temperature

Storage: at -20°C for 24 months

Phagen Lambda DNA