Description/Preparation: 
The molecule is double-stranded circle, 4361 bp in length. pBR322 contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol. The molecular weight of pBR322 is 2.83x10⁶ daltons. pBR322 is isolated from E. coli strain HB101 by a standard plasmid purification procedure.

Usage:  pBR322 is a commonly used plasmid cloning vector in E. coli

Concentration:    0.5 µg/µl

Storage Buffer: 10 mM Tris-HCl (pH 8.0), and 1 mM EDTA.

Quality control: Gel analysis for purity. HinfI and AluI fragmentation patterns.
 
Transportation: Shipped on blue ice or room temperature

Storage: at -20°C for 24 months

pBR322