Dimethylsulfoxide

Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals (Molecular Biology Grade)

Synonym	DMSO, Methylsulfoxide
State of matter	Liquid
Density	1.103 kg/l (20°C)
n 20°C/D	1.478 - 1.480
Melting point	18°C
Boiling point	189°C
Formula	C₂H₆OS
Mass	78.13 g/mol
CAS-No.:	67-68-5

EC-No.:	200-664-3
Storage:	RT
LGK:	10 - 13
Disposal:	1
WGK:	1
Specification
Assay (GC)	min. 99.5 %
Free acid	max. 0.001 %
Non-volatile matter	max. 0.001 %
Heavy metals (as Pb)	max. 0.001 %
Insoluble matter	passes test
Water (K.F.)	max. 0.1 %
A (1 cm/water HPLC grade)
280 nm	max. 0.25

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Deutsche Beschreibung

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References / Protocols / Notes / Recomendations / Tests

  (1)  Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition; pages 1.79-1.81, 2.68; Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
  (2)  Seto, D. (1990) Nucleic Acids Res. 18, 5905-5906
An improved method for sequencing double stranded plasmid DNA from minipreps using DMSO and modified template preparation.
  (3)  Sidhu, M.K. et al. (1996) BioTechniques 21, 44-47
Dimethylsulfoxide increases the RNA amplification.
  (4)  Kelly, R.B. & Sinsheimer, R.L. (1967) J. Mol. Biol. 29, 229-236
Examination of the replication of bacteriophage MS2.
  (5)  Spencer, R.L. & Wold, F. (1969) Anal. Biochem. 32, 185-190
Simple method for the estimation of the cysteine / cystine content in proteins.

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Dimethylsulfoxide