BCIP - 5-Bromo-4-chloro-3-indolyl phosphate
For the colorimetric detection of alkaline phosphatase-labeled molecules.
5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. (Molecular Biology Grade)
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Stability tests / Quality control / Comparison
5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. (Molecular Biology Grade)
Final price excl. shipping costs3
- verfügbar / avaílable
- 3 - 5 days for delivery / 3 - 5 Tage Lieferzeit1
Deutsche Beschreibung
Datasheet
Material Safety Datasheet
References / Protocols / Notes / Recomendations / Tests
(1) Horwitz, J.P. et al. (1966) J. Med. Chem. 9, 447
Substrate for cytohistochemical determination of enzyme activities: Dihalo-3-indolylphosphates.
(2) Gossrau, R. (1978) Histochemistry 58, 203-218
Comparison of the sensitivity of different hydrolase substrates for their histochemical determination.
(3) Leary, J.J. et al. (1983) Proc. Natl. Acad. Sci. USA 80, 4045-4049
Bio-blots for DNA and RNA with BCIP as a substrate/phosphatase linked antibody.
(4) Ey, P.L. & Ashman, L.K. (1986) Methods Enzymol. 121, 497-509
Determination of alkaline phosphatase connected antibodies in immunoblots.
(5) Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) (1995) Current Protocols in Molecular Biology.
Supplement 66, Page 10.8.17. Greene Publishing & Wiley-Interscience, New York.
Contact
GeneOn GmbH
Paracelsusstrasse 10
67071 Ludwigshafen am Rhein
Deutschland / Germany
Phone: +49-621-5720 864
Fax: +49-621-5724 462
Shop-Terms
AGB / Businesss Condtitions / Freight Costs / Frachtkosten für Deutschland
See Categories
- DNA/RNA Extraction
- Real-time PCR
- RT-qPCR - Master Mixes
- PCR / DNA Amplification
- RT-PCR - Reverse Transcription
- Nucleotides, dNTPs, Primers
- Marker / Gel Staining.
- Enzymes and Fine chemicals
- Fluorescent Dyes / supplements
- DNA/Ladders/Standards
- PCR-96 plates and tools, Tips, Tubes, Filtration
- Restriction Endonucleases
- Devices and Tools
News & Informations
We accept payment through
GENEON GmbH - Germany - Paracelsusstrasse 10 - 67071 Ludwigshafen - www.geneon.net - info(at)geneon.net - Tel: 0621-5720864