First Strand cDNA PLUS Synthesis Kit

Features:

•  for synthesis of cDNA from 100 bp up to 10 kb length
•  higher cDNA yield and greater efficiency for full length cDNA synthesis compared with standard M-MLV RT
• increased thermostability

Description:
The First Strand cDNA Plus Synthesis kit contains all reagents for first strand cDNA synthesis and offers a simple handling with high flexibility. A genetically engineered version of M-MLV Reverse Transcriptase with no RNase H activity is a RNA-directed DNA enzyme that synthesizes a complementary DNA strand initiating from a primer using single-stranded RNA or DNA as template.

Applications:
- Synthesis of highly structured and long cDNAfragments
- extremely sensitive and highly specific RTPCR
- DNA labeling

Components:
- Reverse Transcriptase (200 U / µl)
- 5 X Complete RT Buffer
- dNTP Mix (10 mM each)
- Oligo-(dT)₂₀ primer (100 µM)
- Random Hexamers (100 µM)
- RNase Inhibitor (40 U / µl)
- RNase-free Water

- Positive Control RNA (10 ng/µl)

QC-Tests:
- Purified free of endo- and exodeoxyribonucleases, phosphatases and
  ribonuclease
- Activity and stability tested in first strand cDNA synthesis

Storage:
Store product @ -20°C. Avoid frequent thawing and freezing

Transport:
Product will be send with "blue ice"

Manufactured and quality-controlled in accordance with ISO 9001:2000

Protocols:
Note: 
For many standard combinations of RNA and primers heat treatment may be omitted with no negative effect on results. A sample denaturation is particularly recommended for RNA targets that:
- exhibit a high degree of secondary structure
- self- or cross-complementary primers
- initial experiments with new targets

The Manual / Datasheet includs protocols for:

1.a) Assay set-up with sample denaturation (RNA/primer with a high degree of secondary structure)

1.b) Assay set-up without sample denaturation (standard RNA/primer combinations)

2. First-strand cDNA synthesis