FAST 2.0 Bst DNA Mastermix 2x with SybrGreen
Isothermal Amplification for sensitive and fast screening of DNA, LAMP (Loop-mediated Isothermal Amplification), chain displacement reaction etc.
Applications:
FAST 2.0 Bst Mastermix is an extraordinary candidate for isothermal amplifications:
- DNA strand displacement amplification (SDA)
- Loop-mediated isothermal amplification of DNA
(LAMP)
- Sequencing of very low amounts of DNA template
Description:
FAST 2.0 Bst Mastermix (2x), genetically improved Bacillus stearothermophilus cloned to E. Coli in a ready-to-use Mastermix, for next generation of isothermal DNA amplification.
The Mastermix allows the detection within up to 5 to 10 minutes (when using an extra primer pair) and thus it is 2 to 3 times quicker than other Bst DNA Polymerases.
The optimized FAST 2.0 Bst Mastermix amplification results can be compared with about 28-31 cycles in a standard PCR-Cycler Platform. The FAST 2.0 Bst Mastermix offers
high strand displacement capability. The optimal temperature range is between 60°C and 65 °C.
Content:
Fast 2.0 Bst Polymerase, dNTPs, reaction buffer, glycerol, SybrGreen as intercalating dye, stabilizer and enhancer.
READY-to-use. The Mastermix is 2x concentrated
The Mastermix cab be combined with ROX reference dye as normalization dye.
Concentration: 2 x
Storage condition: @ -20°C; for short term (up to 10-12 weeks) at +4°C
Shipping: on blue ice
References / Protocols / Notes / Recomendations
Protocol for 50 µl reaction volume
| Component |
Concentration final |
Volume |
|
FAST 2.0 Bst Mastermix Evagreen dye with or without ROX; (10x) |
1x | 25 µl |
| Primer Mix (10x) | 1x | 5 µl |
| Template DNA | max. 500 ng | X µl |
| PCR-Water | up to 50 µl |
BST Mastermix is available with Evagreen product-code S650, or Evagreen and High ROX product-code S660
FAST 2.0 Bst Mastermix is an extraordinary candidate for isothermal amplifications:
- DNA strand displacement amplification (SDA)
- Loop-mediated Isothermal Amplification
Final price excl. shipping costs3
- verfügbar / avaílable
- 3 - 5 days for delivery / 3 - 5 Tage Lieferzeit1
1. A LAMP Primer Mix can be prepared with all 4 or 6 (with Loop) primers. A 10X Primer Mix should contain: 16 µM FIP, 16 µM BIP, 2 µM F3, 2 µM B3, 8 µM LoopF, 8 µM LoopB in TE or water
2. Reactions should be setup on ice.
3. If analyzing via agarose gel electrophoresis or other method requiring opening LAMP reaction vessels, setup secondary analysis area and equipment to avoid contamination.
4. Running a no-template control is strongly recommended to ensure amplification specificity.
5. If optimization is desired, try titrating Mg2+ (4–10 mM final) or Bst DNA Polymerase, Large Fragment (0.01–0.2 U/µl), or changing reaction temperature (50–68°C).
6. dNTP should be used with dTTP, not dUTP
7. We recommend using LAMP primer design software such as Primer Explorer (http://primerexplorer.jp/e/).
Deutsche Beschreibung
Bst DNA Polymerase
Datasheet Fast 2.0 Bst DNA Polymerase
READY-to-use.
Material Safety Datasheet
Contact
GeneOn GmbH
Paracelsusstrasse 10
67071 Ludwigshafen am Rhein
Deutschland / Germany
Phone: +49-621-5720 864
Fax: +49-621-5724 462
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