Magnetic Bead Plant Genomic Isolation DNA Kit

Sample Preparation

1. Cut off 100 mg of the fresh plant tissue or 50 mg of the dry plant tissue.

2. Grind the sample in the liquid nitrogen to a fine powder using a mortar and pestle.

3. Add 400 μl of the Grind Buffer to the pestle and mortar and continue to homogenize the

sample tissue by grinding.

Step 1 Lysis

1. Transfer the mixture from the Grind Step to a 1.5 ml microcentrifuge tube.

2. Incubate at 70°C for 30 minutes to lyse the sample. During incubation, invert the tube

every 5 minutes.

3. Centrifuge for 5 minutes at 5,000 x g.

4. Transfer the supernatant to a new 1.5 ml microcentrifuge tube and add 200 μl of Lysis

Buffer. Mix well and incubate at 65°C for 5 minutes. During this time, pre-heat the

Release Buffer to 65ºC for Step 4.

5. Add 400 μl of the isopropanol to the lysate and mix well.

Step 2 DNA Binding

1. Add 20 μl of the Magnetic Beads. Mix well by gently shaking for 3 minutes.

2. Place the tube in a magnetic separator for 30 seconds.

3. Remove the solution (If the mixture becomes viscous, increase magnetic bead separation

time).

Step 3 Wash

1. Add 800 μl of the Wash Buffer and mix well (Following the wash, the mixture will no

longer be viscous).

2. Place the tube in a magnetic separator for 30 seconds. Remove the solution.

Step 4 Release

1. Add 200 μl of the Release Buffer (pre-heated to 65ºC) and mix well.

2. Incubate for 3 minutes at 65ºC (during incubation, shake the tube vigorously every

minute).

3. Place the tube in a magnetic separator for 1 minute.

4. Carefully transfer ONLY the clean portion of the solution to a clean tube.

NOTE: Be sure and allow the magnetic beads to disperse completely during the binding, wash and elution