Magnetic beads for high-throughput isolation and extraction of DNA and RNA (nucleic acids)

Please have a look to complete "MBSi" reagent isolation kits for nucleic acid samples, including clinical material like Oropharyngeal swabs, Nasopharyngeal swabs, Sputum for:

• Manual use in tubes or plates
  
  
• automatically method in plates for:  KingFisher Flex, Thermo Fisher Scientific and Auto-Pure 96 from Hangzhou Allsheng Instruments Co., Ltd or similar
  
  
• automatically method fo Freedom EVO® series, TECAN Schweiz AG or similar

We recommend using neodymium magnets or a special magnetic tripod for effective separation.

The principle of the "MBSi" Magnetic Beads sorbent  is based on the reversible binding of nucleic acid molecules on the surface of magnetic sorbent particles. The sample is treated with a lysis solution in the presence of magnetic sorbent particles. As a result, cell membranes, viral membranes and other biopolymer complexes are destroyed and nucleic acids are released.

Dissolved NA links with the sorbent particles, while the other components of the lysed biological material remain in solution and are removed during magnetic precipitation of sorbent on a magnetic stand followed by washing of sorbent.

When an elution buffer is added to a magnetic sorbent, NA is eluted from the surface of the sorbent into solution, which is then separated from the sorbent particles by magnetic force. As a result of this procedure, a highly purified NA sample is obtained, free from amplification reaction inhibitors, which ensures high analytical sensitivity of the RT-PCR study.