Problem

Cause

Solution

DNA is sheared or degraded

Lysate mixed too vigorously

Use the appropriate pipette tip set for the required volume, lower it under the reading line of the solution to mix the sample, pipet up and down gently to mix.

DNases contamination

Maintain a sterile environment while working (e.g. wear gloves and use DNase-free reagents).

RNA containment

Incomplete removal of the

RNAse

RNase A treatment

Low yields of gDNA

Incomplete lysis and homogenization

Complete lysis.

Use the appropriate method for the lysate preparation based on the amount of starting materials.

Incorrect handling of

Magnetic Beads

Vortex the tube containing the Magnetic Beads to fully resuspend the beads before adding them to your sample.

Incorrect elution conditions

Add the Release Buffer (50~100µl ) and incubate for 3 min at 65°C.

The quality of the starting material may not be optimal

Use fresh sample and process immediately after collection, or freeze the sample at -80°C or in the liquid nitrogen.

High background on UV measurement

Residual beads released

Repeat the magnetic separation and transfer the eluate to a clean tube.