DFS-PLUS "Hot"-Taq DNA Polymerase; Hot-Start blocked with MAB

Features:
DFS-Taq "Hot" is the " Hot Start PCR " version of DFS Taq DNA Polymerase that provides a new formula in buffers and additives to prevent failures in PCR-applications were inhibitors (e.g. proteins, fat or PS) reduce the performance.

The Taq Polymerase is blocked with monoclonal Antibodies. Both components, the Taq Polymerase and the Antibody is available, separatly at GeneON's shop.

The robust hot start polymerase is well suited for sensitive experiments using random primers or bacterial templates. Because of the high sensitivity less than 6 molecules can be detected.

• reliable and reproducable quantification in qPCR
• perfect for real time PCR
• especially for diagnostic purposes
• reaction set-up at room temperature
• activation of enzyme during first heating
• no change or optimization of protocol necessary
• high specifity, reduced primer mismatch or dimers

Application:
Instead of conventionally purified Taq-DNA Polymerase for sensitive PCR reactions, for the detection of bacterial DNA or for applications where inhibitors decrease the performance of regular polymerases.

• Hot start PCR
• Real time PCR
• Amplification of complex genomic and cDNA templates
• Multiplex PCR
• High specifity PCR

Reaction conditions:
Same as for conventionally purified Taq-DNA Polymerase.

Concentration
5 units/μl supplied in 10 mM KPO₄ (pH 7.4 at 25°C), 0.1 mM EDTA, 0.1% Tween 20, 0.1% Triton-X 100 and 50 % (v/v) glycerol.

Unit Definition
One unit is defined as the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble material in 30 min at 74°C.

Reaction Buffers provided:
Ammonium-Reaction buffer (10X)” incomplete” 
Ammonium-Reaction buffer (10X) “complete” with 25mM MgCl2
MgCl2 (100 mM)

Transport: with "blue ice" or at ambient temperature.

Storage: at –20 °C is recommended to safeguard against growth of bacteria that may be introduced during handling.

Quality Control:
- Endonucleases Incubation of 20 units of the enzyme in 1x reaction buffer
  with 1 μg lambda DNA for 16 h
  at 65°C in 50 μl yields no detectable degradation of DNA.
- Incubation of 20 units of the enzyme in 1x reaction buffer with 1 μg
  lambda DNA EcoR I/Hind III fragments
  for 16 h at 65°C in 50 μl yields no detectable degradation of DNA.
- Incubation of 32 units of the enzyme in 1x reaction buffer with 1 μg
  supercoiled pUC18 DNA for 16 h at 70° C in 50 μl resulted in no
  relaxation.
- Priming activity Incubation of 40 units of the enzyme in 1x reaction buffer
  with 100 ng template DNA and  
  0.2 mM dNTPs each, but without primers in 100 μl resulted in no DNA
  synthesis.
- PCR Test Good performance of DNA amplification was confirmed by
 using Lambda DNA as template (amplified fragment 12 kb) and human
 placenta DNA as template (amplified fragment 3.0 kb).
- No DNA contamination with enterobacterial DNA

Note: 
- vortex all solutions carefully before using
- dispense all reagents on ice
- add the enzyme after Template DNA
- may you have to optimize the MgCl₂ concentration for best result

General Thermo-Cycler protocol:

Dear customer, GeneON likes to send free samples to convince the valued customer about the quality. Please understand that the shipping costs may be very high to some destinations. That is the reason why we cannot assure to fulfil all sample requests. Sorry for that, please understand. You may also set an inquiry/order directly by e-mail .