COT I Human DNA (blocking reagent)
Cot-1 human DNA for: In situ hybridisation, micro-array, DNA array comparative genomic Hybridisation (CGH), and genetic analysing
Applications:
- In situ suppression (CISS) hybridizations
- Hybridization to micro-arrays
- Other In situ hybridizations
- Filter hybridizations
Description:
COT I Human DNA is prepared from human placental DNA by shearing, denaturing, and reannealing under conditions that enrich these repetitive elements.
The product is prepared from male human placental DNA, exclusively.
The COT I DNA fraction of human genomic DNA consists largely of rapidly annealing repetitive elements. These interspersed repetitive sequences (IRS), such as SINEs (small interspersed repetitive
elements, e.g., Alu elements) and LINEs (large interspersed repetitive elements, e.g., L1 elements),are distributed ubiquitously throughout the genome.
Concentration: > 1,1 mg/ml; Solution in 10 mM Tris-HCl, 1 mM EDTA, pH 7.4
The COT I DNA is available in concentration greater than 10 mg/ml (product code 3005)
Quality
control:
- Average fragments size: 50-300 bp
- A260/A280 ration: about 1.70
- Amount of genomic (non-repetitive DNA): less than 5%
- COT I DNA and the raw material is tested for the absence of HIV1,2 RNA, HCV RNA, HBV DNA
Transportation: on blue ice
Storage: at -20°C for more than 12 months
Stability tests / Quality Tests: Cot I DNA
COT I Human DNA is available in several concentrations.
Cot-1 DNA for: In situ hybridisation, micro-array, DNA array comparative genomic Hybridisation (CGH), and genetic analyzing.
Cot I human DNA is available with a concentration of 1,1 mg/ml (product.-no. 3001) or with a concentration of up to 10 mg/ml with product code number 3005.
* availibility of sample size may be limited
Final price excl. shipping costs3
- verfügbar / avaílable
- 1 - 3 days for delivery / 1 - 3 Tage Lieferzeit1
Free sample request Cot 1 human DNA
Dear customer, GeneON likes to send free samples to convince the valued customer about the quality. Please understand that the shipping costs may be very high to some destinations. That is the reason why we cannot assure to fulfil all COT I human DNA sample requests. Sorry for that, please understand. You may also set an inquiry/order directly by e-mail .
Datasheet Cot I human DNA
Material Safety Datasheet
References / Protocols / Notes / Recomendations / Tests
Recommendation from a researcher:
For Southern blot hybridizations: add 50 μg of COT-1 human DNA (@ 10 μg/μL) to 50 μL of 20X SSC, 25 μL distilled water and 20 μL of a solution containing 0.1 M NaCl, 0.1 M
Tris-HCl (pH 7.4). 0.01 M EDTA, and 1% SDS to the probe for each 25 to 500 ng of probe.
For in situ hybridizations: combine genomic probe with the proper amount of COT-1 human DNA such that the final concentration of COT-1 human DNA is 0.3 μg/μL for cosmid, plasmid,
and lambda probes; or, at 1 μg/μL for Alu PCR probes.
Ethanol precipitate and resuspend in a half-volume of 100% formamide. Add a half-volume of 20% dextran sulfate in 2X SSC (prewarmed to 75 degrees C) and mix well. Denature mix by heating to 75 °C
for 5 min. Incubate at 37 °C for at least 5 and up to 15 min.
Interesting links:
Anal Chem (2002) 74:1792-1799
Product usage: Superscript II RT was used to make labeled cDNA from a human polyA RNA sample (42C incubation temp). Cy3 or Cy5 labeled dCTP was used for these experiments. Remaining RNA was degraded by using a combination of RNase H (Invitrogen) and RNase A (USB). Array hybridization was per......
J Biol Chem (1996) 271:28324-28354
Product usage: Cultures of the human squamous cell carcinoma line SCC12(c12c2) were plated at 2.5 × 106 to 5 × 106 cells/60-mm dish and grown to 70-90% confluence before transfection. The transient transfection was performed by a lipofection method in a serum-free medium with 2.5 µg of each pla......
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