Bovine Serum Albumin - BSA nativ - non-acetylated

Τhis product contains >99% Albumin, it is free of proteases, fatty acids and contains extremely low heavy metal ions.

Free of RNase/DNase.

It is also free of DNA, RNA and nonspecific endonuclease, exonuclease and RNase activity as determined after incubation with appropriate DNA and RNA substrate and analysed by agarose gel electrophoresis.

Description:
Bovine Serum Albumin (BSA) is supplied with some restriction enzymes to prevent adhesion of the enzyme to reaction tubes and pipette surfaces. BSA also stabilizes some proteins during incubation. The source of purified BSA is a molecular biology grade, protease free, fraction V starting material. This material is then processed to remove nucleases.

Concentration: 10 mg/ml (or other concentration up to 50mg/ml on request)

Storage Buffer: Dest. Water or

in Glycerol-NEB-Buffer: 20 mM KPO₄, 50 mM NaCl, 0.1 mM EDTA, 5% Glycerol, pH 7.0 (25°C)

Storage Temperature: -20°C

Quality Control:
A 16 hour incubation is performed with ΦX174 DNA- HaeIII Digest. This assay checks for endonuclease, exonuclease and binding protein contamination. 16 hour incubation is performed with ΦX174 RFI DNA, as sensitive assay for nonspecific endonuclease and 1 hour incubation with a RNA ladder is performed. Produced in ISO-certificated Company.

Transport: Ambient or with blue ice

Note:
- It is do not recommend using this preparation of BSA as a standard for
  quantitation purposes or size determination.
- The BSA should be diluted 1:100 for standard reactions. At this level the
   buffer from the BSA have a very small effect, only.