Description

The Tissue Blood Combi DNA Extraction Kit is designed for rapid and efficient purification of genomic DNA from blood and various fresh or frozen tissue samples such as kidney, heart, lungs, brains, muscles, liver, spleen, and animal cultured cells. The purification is based on the usage of denaturing agents to provide lysis of cells, denaturation of proteins and subsequently release of genomic DNA. Special buffers provided in the kit are optimized to enhance binding of DNA onto a specially-treated glass filter membrane for efficient recovery of highly pure genomic DNA. Simple centrifugation steps allow multiples sample processing and remove contaminants to yield highly pure DNA which is suitable to be used in various down-stream applications.

Features

• Yields up to 20µg of DNA

• No organic-based extraction required.

• Highly pure genomic DNA ready to use for routine molecular biology applications such as restriction enzyme digestion, PCR, Southern Blotting and DNA fingerprinting.

Kit Components

- Buffer TL

- Lysis Enhancer

- Buffer CB

- Wash Buffer 1 (concentrate)

- Wash Buffer 2 (concentrate)

- Elution Buffer

- Proteinase K

This Product has been used In:

El-Gohary, R., Wagih, A.A., Hamouda, H.E., El-Melegy, S.M., Rowisha, M.A. (2018). Association of STAT6 rs324011 Gene Polymorphism with Susceptibility of Atopic Bronchial Asthma in Egyptian Children, Biochemistry and Molecular Biology Journal, Vol. 4, No. 1:6 (2018).

Abdossamadi, Z., Seyed, N., Zahedifard, F., Taheri, T., Taslimi, Y. (2017) )Human Neutrophil Peptide 1 as immunotherapeutic agent against Leishmania infected BALB/c mice. . PLOS Neglected Diseases Vol. 11, No. 12 (2017).

Dear customer, GeneON likes to send free samples to convince the valued customer about the quality. Please understand that the shipping costs may be very high to some destinations. That is the reason why we cannot assure to fulfil all sample requests. Sorry for that, please understand. You may also set an inquiry/order directly by e-mail .