COT I Human DNA acc. Fluoro.

Applications:

Array CGH, Cell Analysis, Cellular Imaging, ChIP-on-Chip, Chromatin Biology, Fluorescence In Situ Hybridization, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, In Situ Hybridization (ISH), Microarray Analysis, RNAi, Epigenetics and Non-Coding RNA Research

 
Description:
COT I Human DNA is prepared from human placental DNA by shearing, denaturing, and reannealing under conditions that enrich these repetitive elements. The product is similar to Invitrogen #15279.
The product is prepared from male human placental DNA, exclusively.
The Cot-I DNA fraction of human genomic DNA consists largely of rapidly annealing repetitive elements. These interspersed repetitive sequences (IRS), such as SINEs (small interspersed repetitive elements, e.g., Alu elements) and LINEs (large interspersed repetitive elements, e.g., L1 elements),are distributed ubiquitously throughout the genome.

Concentration: 1,1 mg/ml; Solution in 10 mM Tris-HCl, 1 mM EDTA, pH 7.4

Quality control:
see below

Transportation: on blue ice

Storage: at -20°C for more than 12 months

Parameter Range                                                          value Test results

Appearance clear, colourless solution                                                            corresponds

Concentration (abs. 260 nm; 0.9 –1.2 mg/ml                                  1,11 mg/ml

in 50mM NaOH)

Ratio C(OD260/)C(Hoechst) ≤1,5                                                      1,45

Non-COT 1 DNA < 5% w/w                                                                   corresponds

Y-Chromosome obtained exclusively from male

human placenta                                                                                     corresponds

 Determination of quotient A260/A280: 1.6 – 2.0                             1.82

HIV1,2, HCV and HBV RNA/DNA not detectable

( PCR/RT-PCR)                                                                                      corresponds

Gel electrophoretic separation in 1.2 % agarose

gel 2.0,3.0 µg without RE cleavage middle chain

length: 50 – 300 bp                                                                             corresponds

Treated by phenol in the process of production                         corresponds

Buffer                                                         10mM TrisHCl, 1mM EDTA, pH 7,4

Dear customer, GeneON likes to send free samples of Cot I DNA accurate to convince the valued customer about the quality. Please understand that the shipping costs may be very high to some destinations. That is the reason why we cannot assure to fulfil all sample requests. Sorry for that, please understand. You may also set an inquiry/order directly by e-mail .