Ahl I - A↑CTAGT - TGATC↓A

Activity in SEBuffers:

SEBuffer B              100%

SEBuffer G       75 -100%

SEBuffer O       25 -  50%

SEBuffer W       25 -  50%

SEBuffer Y        75 -100%

SEBuffer ROSE      100%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 37°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol;

Store at -20°C.

Ligation: After 20-fold overdigestion with enzyme more than 90% of T7 DNA fragments can be ligated and recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 40 u.a. of enzyme for 16 hours at 37°C.

Reagents Supplied:with Enzyme 10 X SE-buffer B, BSA 

Methylation sensitivity: not tested

Inactivation 20 minutes: No

Notes: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.