Acl I - AA↑CGTT - TTGC↓AA

Activity in SEBuffers:

SEBuffer B      0 -  10%

SEBuffer G      0 -  10%

SEBuffer O      0 -  10%

SEBuffer W      0 -  10%

SEBuffer Y           100%

SEBuffer ROSE    80%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 37°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 0.05% Triton X-100; 50% glycerol;

Store at: -20°C.

Ligation: After 2-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C.

Reagents supplied with enzyme: 10 X SE-buffer Y, BSA

Methylation sensitivity: Blocked by CG methylation

Inactivation: 20 minutes under 65°C

Notes: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.