Activity in SEBuffers:

SEBuffer B       25 - 50%

SEBuffer G       50 - 75%

SEBuffer O       75 -100%

SEBuffer W             100%

SEBuffer Y         50 - 75%

SEBuffer ROSE       100%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 37°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol.

Store: at -20°C.

Ligation: After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.

Reagents Supplied with Enzyme: 10 X SE-buffer W

Methylation sensitivity: Blocked by overlapping Dam methylation (GmATC) GATC .

Not blocked by CG methylation.

Cut hemimethylated site: 5`- GmATC-3` / 5`-GATC-3`

Inactivation: 20 minutes under 65°C