Mfe I - C↑AATTG - GTTAA↓C

Activity in SEBuffers:

SEBuffer B              100%

SEBuffer G       75 -100%

SEBuffer O        10 - 25%

SEBuffer W       25 - 50%

SEBuffer Y       75 -100%

SEBuffer ROSE        25%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 37°C

Storage conditions: 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol.

Store at: -20°C.

Ligation: After 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated with T4 DNA Ligase and recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.

Reagents supplied with enzyme: 10 X SE-buffer B, BSA

Inactivation: 20 minutes no

Notes: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.